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And then biotinylated Human NPNT Antibody is added and binds to NPNT in the sample
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Apln in the sample
Substrate solution is then added and color develops in proportion to the amount of Human DKKL1
The concentration of S100A7 in the sample was calculated by drawing a standard curve
Rat ANG1 (Angiopoietin-1) ELISA Kit CINC-1 And then biotinylated Human NPNTThe Rat ANG1 (Angiopoietin 1) ELISA Kit measures ANG1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ANG 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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