Rat RGN (Regucalcin) ELISA Kit Packaging:96 wells Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Canine CALCA

Only those wells that contain Mouse TGF-Alpha

Then a biotinylated detection antibody specific for Mouse MIP-1Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then a biotinylated detection antibody specific for Mouse ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Rat RGN (Regucalcin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat RGN (Regucalcin) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat RGN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RGN and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and