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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFa5
The plate has been pre-coated with Mouse DKK1 antibody
Then a biotinylated detection antibody specific for Human LDHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated ALDH3A1 antibody
Human SERPINB13 (Serpin B13) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (SERPINB13) Serpin B13 ELISA Kit measures Serpin B13 in samples. The plate has been pre coated with Human SERPINB13 antibody. SERPINB13 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SERPINB13 Antibody is added and binds to SERPINB13 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SERPINB13 antibody. After incubation unbound Streptavidin HRP is washed away
