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the remaining conjugate is allowed to react with the substrate solution (TMB)
You can calculate the concentration of Human IL-1R2 in the samples by comparing the OD of the samples to the standard curve
then E2 enzyme conjugate is added on the top of the reaction mixture and incubation continues for 45 minutes more
Then Streptavidin-HRP is added and binds to the Biotinylated S100A8 antibody
Human ALK (Anaplastic lymphoma kinase) QuickTest ELISA Kit AD10 the remaining conjugate is allowedThe Human ALK (Anaplastic lymphoma kinase) QuickTest ELISA Kit measures ALK in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
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