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Substrate solution is then added and color develops in proportion to the amount of Human AMELY
The concentration of Mouse TUBb in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Rat CD36 antibody
only those wells that contain Rat HNE
Rat PDYN (Proenkephalin-B) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat (PDYN) Proenkephalin B ELISA Kit measures Proenkephalin B in Rat samples. The plate has been pre coated with Rat PDYN antibody. PDYN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PDYN Antibody is added and binds to PDYN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PDYN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.