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The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 36 nm
The plate has been pre-coated with Human BRCC3 antibody
And then biotinylated Human GOLGA2 Antibody is added and binds to GOLGA2 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SOD1
Human GSH (Glutathione) ELISA Kit THPH8 The optical density (OD) isThe Human (GSH) Glutathione ELISA Kit measures glutathione in samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color
