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The plate has been pre-coated with Mouse AMPD2 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated PGLYRP3 antibody
The concentration of Mouse FIL1d in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human WNT5B (Protein Wnt-5B) ELISA Kit Packaging:96 wells The plate has been pre-coatedThe Human (WNT5B) Protein Wnt 5B ELISA Kit measures Protein Wnt 5b in samples. The plate has been pre coated with Human WNT5B antibody. WNT5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WNT5B Antibody is added and binds to WNT5B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated WNT5B antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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