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Porcine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg
Then a biotinylated detection antibody specific for Rat AGER and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human CARD9 antibody
Substrate solution is then added and color develops in proportion to the amount of Human MGP
Human CLU (Clusterin) ELISA Kit Packaging:96 wells Porcine Pg in samples (orThe Human CLU (Clusterin) ELISA Kit measures CLU in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CLU antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,
