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Calculate the concentration of HCAR1 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated SYT16 antibody
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Human NES1 (Nesfatin 1) ELISA Kit Packaging:96 wells Calculate the concentration of HCAR1The Human NES1 (Nesfatin 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human NES1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NES1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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