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a capture antibody-TIMP-3-biotin-detection antibody complex was formed
ENTPD1 present in the sample is added and binds to antibodies coated on the wells
The microplate provided in this kit has been precoated with anti HMGB4 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated VIPR1 antibody
Human AlphaMSH (Alpha-Melanocyte Stimulating Hormone) ELISA Kit Packaging:48 wells a capture antibody-TIMP-3-biotin-detection antibody complexThe Human AlphaMSH (Alpha Melanocyte Stimulating Hormone) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human AlphaMSH. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Human AlphaMSH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB
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