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The concentration of FGF20 in the sample was calculated by drawing a standard curve
GLO1 present in the sample is added and binds to antibodies coated on the wells
The concentration of NLRC4 in the sample was calculated by drawing a standard curve
And then biotinylated Bovine OPN Antibody is added and binds to OPN in the sample
Human FBLN5 (Fibulin 5) ELISA Kit NIF The concentration of FGF20 inThe Human FBLN5 (Fibulin 5) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human FBLN5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBLN5 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
