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And then biotinylated Human KLF4 Antibody is added and binds to KLF4 in the sample
FGL1 present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Human HMOX1 antibody
The concentration of VIP in the sample was calculated by drawing a standard curve
Rat CCN5 (CCN family member 5) ELISA Kit Packaging:96 wells And then biotinylated Human KLF4The Rat CCN5 (CCN family member 5) ELISA Kit measures CCN5 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CCN5 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
