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Substrate solution is then added and color develops in proportion to the amount of Human SYT3
Then a biotinylated detection antibody specific for Human VF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
You can calculate the concentration of Mouse gp130 in the samples by comparing the OD of the samples to the standard curve
The concentration of Human MYH3 in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse CNTF (Ciliary neurotrophic factor) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Mouse CNTF (Ciliary neurotrophic factor) ELISA Kit measures Cntf in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Cntf antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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