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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat TNFa
Substrate solution is then added and color develops in proportion to the amount of Equine TLR4
The concentration of Human IL1h in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rabbit NUP214 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Guinea pig MIF (Macrophage Migration Inhibitory Factor) ELISA Kit pp1158 The microtiter plate provided inThe Guinea pig MIF (Macrophage Migration Inhibitory Factor) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig MIF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig MIF and Avidin Horseradish Peroxidase (HRP) conjugate are added
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