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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MUC1
Then a biotinylated detection antibody specific for Mouse RETN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
C6orf105 present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Human CD151 antibody
Human CST5 (Cystatin-D) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (CST5) Cystatin D ELISA Kit measures Cystatin D in samples. The plate has been pre coated with Human CST5 antibody. CST5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CST5 Antibody is added and binds to CST5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CST5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
