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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TNFSF13
The assay plate has been pre-coated with mouse anti-Mouse CD5L monoclonal antibody
The microtiter plate is coated with the antibody specifically binding Insulin-like Growth Factor-binding Protein 7
The concentration of Human CHI3L1 in the samples is then determined by comparing the OD of the samples to the standard curve
Human AZU (Azurocidin) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (AZU) Azurocidin ELISA Kit measures Azurocidin in samples. The plate has been pre coated with Human AZU1 antibody. AZU1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AZU1 Antibody is added and binds to AZU1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated AZU1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
