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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRF1
The Rat (DDIT3) DNA Damage-inducible Transcript 3 Protein ELISA Kit measures DNA damage-inducible transcript 3 protein in Rat samples
Then a biotinylated detection antibody specific for Mouse HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Rabbit IL-5 ELISA kit is a sandwich enzyme immunoassay
Human NTN4 (Netrin-4) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (NTN4) Netrin 4 ELISA Kit measures Netrin 4 in samples. The plate has been pre coated with Human NTN4 antibody. NTN4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NTN4 Antibody is added and binds to NTN4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NTN4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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