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The concentration of Mouse HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve
the Human Cyt-C (Cytochrome C) ELISA kit uses the Sandwich-ELISA principle
Then a biotinylated detection antibody specific for Mouse C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then a biotinylated detection antibody specific for Human SEMA3A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human CP (Ceruloplasmin) ELISA Kit KSP3 The concentration of Mouse HMGB-1The Human (CP) Ceruloplasmin ELISA Kit measures Ceruloplasmin in samples. The plate has been pre coated with Human CP antibody. CP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CP Antibody is added and binds to CP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CP antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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