HaXS8 cy3 66 SMILES CC1=C(SC=N1)C1=CC=C(CNC(=O)[C@@H]2C[C@@H](O)CN2C(=O)[C@@H](NC(=O)CCCO)C(C)(C)C)C=C1 Purity (HPLC)

66 SMILES CC1=C(SC=N1)C1=CC=C(CNC(=O)[C@@H]2C[C@@H](O)CN2C(=O)[C@@H](NC(=O)CCCO)C(C)(C)C)C=C1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

364 SMILES OCCCNC(=O)COC1=C2C(=O)N(C3CCC(=O)NC3=O)C(=O)C2=CC=C1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

318 SMILES CC(C)(C)OC(=O)N1CCN(CCC(O)=O)CC1 Purity (HPLC) >= 95% Shipping Conditions Room temperature Storage Conditions Refrigerated Shelf Life 12 months after the date of delivery Regulatory Statement For Research Use Only

Nature Chemical Biology

HaXS8 cy3 66 SMILES CC1=C(SC=N1)C1=CC=C(CNC(=O)[C@@H]2C[C@@H](O)CN2C(=O)[C@@H](NC(=O)CCCO)C(C)(C)C)C=C1 Purity (HPLC)HaXS8 is a synthetic chemical dimerizer designed to induce covalent and irreversible intracellular dimerization between proteins tagged with HaloTag and SNAP tag. Its a bifunctional molecule featuring a chloroalkane group (specific for HaloTag) and an O6 benzylguanine (BG) derivative (specific for SNAP tag), connected by a flexible polyethylene glycol (PEG) linker. When introduced to cells, HaXS8 bridges HaloTag and SNAP tag fusion proteins by